We've published a new paper
Happy to share our article on Factor Indexing (FI)-snMultiome-seq method by Liangru Fei from our group in NCMM in collaboration with Sampsa Hautaniemi and Kaiyang Zhang from University of Helsinki, Finland in Cell Press STAR Protocols.
Abstract
Ectopic expression of lineage-specific transcription factors (TFs) of another cell type can induce cell fate reprogramming. However, the heterogeneity of reprogramming cells has been a challenge for data interpretation and model evaluation.
Here, we present a protocol to characterize cells expressing defined factors during direct cell reprogramming using a factor-indexing approach based on single-nuclei multiome sequencing (FI-snMultiome-seq). We describe the steps for barcoding TFs, converting human fibroblasts to pancreatic ductal-like cells using defined TFs, and preparing library for FI-snMultiome-seq analysis.
For complete details on the use and execution of this protocol, please refer to Fei et al.
